Description
This module describes some fundamental techniques and procedures that are used regularly in microbiology laboratories.
Course Objectives
Students completing this course should be able to:
- Work effectively in a cleanroom cabinet
- Describe how media is prepared and quality controlled
- Read plates and report results
- State the requirements for microbiology method validations
- Handle and maintain cultures according to laboratory procedures
- State the special requirements and limitations of the sterility test
Course Outline
INTRODUCTION
1. Course Overview
2. Overview
3. Assessment
LAMINAR FLOW AND BIOHAZARD CABINETS
4. Introduction
5. Airflow patterns I
6. Airflow patterns II
7. Transferring materials
8. Review
MEDIA PREPARATION AND QC
9. Introduction
10. Importance of media
11. Media preparation notes
12. QC tests
13. Storage conditions
14. Importance of traceability
15. Review
READING EXPOSURE PLATES
16. Introduction
17. Environmental monitoring
18. Microbiological plating
19. Membrane filtration
20. Direct plate techniques
21. Exposure plate reliability
22. Reading and recording
23. Review
METHOD VALIDATION FOR MICROBIOLOGY
24. Introduction
25. Test methods
26. Typical validation tests
27. Examples
HANDING AND MAINTAINING CULTURES
28. Introduction
29. Why maintain cultures?
30. Sources of cultures
31. Cultures as references
32. Precautions
33. Subculturing requirements
34. Review
THE STERILITY TEST
35. Introduction
36. Purpose & principles
37. Unsatisfactory test results
38. Satisfactory test results
39. Sterility test environment
40. Negative
41. Review
CONCLUSION
42. Summary